heparg human hepatoma cell line Search Results


90
KAC Co Ltd human hepatoma cell line heparg hpr116-ta10
Human Hepatoma Cell Line Heparg Hpr116 Ta10, supplied by KAC Co Ltd, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Biopredic human hepatoma heparg cells
Human Hepatoma Heparg Cells, supplied by Biopredic, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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90
Merck KGaA human hepatocellular carcinoma cell line heparg
Fisetin (FST) attenuates acetaminophen (APAP)-induced cytotoxicity by promoting autophagy and inhibiting inflammasome activation in L-02 cells. (A, B) Cell viability was assessed by MTT assay in response to APAP (10 mM) or N-acetyl-p-benzoquinoneimine (NAPQI) (200 μM) exposure for different periods in L-02 cells. (C, D) Cell viability was assessed by MTT assay in response to APAP (10 mM) or NAPQI (200 μM) exposure for 48 h in <t>HepaRG</t> cells. (E) Cell viability was assessed by MTT assay for different concentration of FST in L-02 cells. (F) Messenger RNA (mRNA) expression of ATG genes was determined by RT-PCR in the presence of APAP (10 mM) for 48 h. (G) Western blot of autophagy-related proteins in L-02 cells supplemented with APAP (10 mM) for 48 h. (H) Quantification of Western blot. (I, J) Representative immunofluorescence images of mRFP-GFP-LC3 in L-02 cells supplemented with APAP (10 mM) for 48 h. Representative profiles of autophagosomes (RFP+GFP+dots) and autolysosomes (RFP+GFP-dots) per cell section, assessed by confocal microscopy are shown and were quantified. (K, L) Autophagic vacuoles (autophagosomes) were assessed by transmission electron microscopy (TEM) plus APAP (10 mM) for 48 h in L-02 cells. Representative TEM images are shown and typical autophagosomes are marked with black arrows. Autophagosome numbers per cell were calculated by counting the number of double-membrane organelles in 10 cells. Data are expressed as mean ± SEM (n = 3). **P < 0.01, ***P < 0.001 compared to control; # < 0.05, ### < 0.001 compared to APAP.
Human Hepatocellular Carcinoma Cell Line Heparg, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/heparg+human+hepatoma+cell+line/pmc07058798-65-2-12?v=Merck+KGaA
Average 90 stars, based on 1 article reviews
human hepatocellular carcinoma cell line heparg - by Bioz Stars, 2026-08
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86
Xenobiotics S.L human hepatoma heparg cells
Fisetin (FST) attenuates acetaminophen (APAP)-induced cytotoxicity by promoting autophagy and inhibiting inflammasome activation in L-02 cells. (A, B) Cell viability was assessed by MTT assay in response to APAP (10 mM) or N-acetyl-p-benzoquinoneimine (NAPQI) (200 μM) exposure for different periods in L-02 cells. (C, D) Cell viability was assessed by MTT assay in response to APAP (10 mM) or NAPQI (200 μM) exposure for 48 h in <t>HepaRG</t> cells. (E) Cell viability was assessed by MTT assay for different concentration of FST in L-02 cells. (F) Messenger RNA (mRNA) expression of ATG genes was determined by RT-PCR in the presence of APAP (10 mM) for 48 h. (G) Western blot of autophagy-related proteins in L-02 cells supplemented with APAP (10 mM) for 48 h. (H) Quantification of Western blot. (I, J) Representative immunofluorescence images of mRFP-GFP-LC3 in L-02 cells supplemented with APAP (10 mM) for 48 h. Representative profiles of autophagosomes (RFP+GFP+dots) and autolysosomes (RFP+GFP-dots) per cell section, assessed by confocal microscopy are shown and were quantified. (K, L) Autophagic vacuoles (autophagosomes) were assessed by transmission electron microscopy (TEM) plus APAP (10 mM) for 48 h in L-02 cells. Representative TEM images are shown and typical autophagosomes are marked with black arrows. Autophagosome numbers per cell were calculated by counting the number of double-membrane organelles in 10 cells. Data are expressed as mean ± SEM (n = 3). **P < 0.01, ***P < 0.001 compared to control; # < 0.05, ### < 0.001 compared to APAP.
Human Hepatoma Heparg Cells, supplied by Xenobiotics S.L, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 86 stars, based on 1 article reviews
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98
Meridian Bioscience bio-76005
Fisetin (FST) attenuates acetaminophen (APAP)-induced cytotoxicity by promoting autophagy and inhibiting inflammasome activation in L-02 cells. (A, B) Cell viability was assessed by MTT assay in response to APAP (10 mM) or N-acetyl-p-benzoquinoneimine (NAPQI) (200 μM) exposure for different periods in L-02 cells. (C, D) Cell viability was assessed by MTT assay in response to APAP (10 mM) or NAPQI (200 μM) exposure for 48 h in <t>HepaRG</t> cells. (E) Cell viability was assessed by MTT assay for different concentration of FST in L-02 cells. (F) Messenger RNA (mRNA) expression of ATG genes was determined by RT-PCR in the presence of APAP (10 mM) for 48 h. (G) Western blot of autophagy-related proteins in L-02 cells supplemented with APAP (10 mM) for 48 h. (H) Quantification of Western blot. (I, J) Representative immunofluorescence images of mRFP-GFP-LC3 in L-02 cells supplemented with APAP (10 mM) for 48 h. Representative profiles of autophagosomes (RFP+GFP+dots) and autolysosomes (RFP+GFP-dots) per cell section, assessed by confocal microscopy are shown and were quantified. (K, L) Autophagic vacuoles (autophagosomes) were assessed by transmission electron microscopy (TEM) plus APAP (10 mM) for 48 h in L-02 cells. Representative TEM images are shown and typical autophagosomes are marked with black arrows. Autophagosome numbers per cell were calculated by counting the number of double-membrane organelles in 10 cells. Data are expressed as mean ± SEM (n = 3). **P < 0.01, ***P < 0.001 compared to control; # < 0.05, ### < 0.001 compared to APAP.
Bio 76005, supplied by Meridian Bioscience, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 98 stars, based on 1 article reviews
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96
Excelitas corp pco_pixelfly
Fisetin (FST) attenuates acetaminophen (APAP)-induced cytotoxicity by promoting autophagy and inhibiting inflammasome activation in L-02 cells. (A, B) Cell viability was assessed by MTT assay in response to APAP (10 mM) or N-acetyl-p-benzoquinoneimine (NAPQI) (200 μM) exposure for different periods in L-02 cells. (C, D) Cell viability was assessed by MTT assay in response to APAP (10 mM) or NAPQI (200 μM) exposure for 48 h in <t>HepaRG</t> cells. (E) Cell viability was assessed by MTT assay for different concentration of FST in L-02 cells. (F) Messenger RNA (mRNA) expression of ATG genes was determined by RT-PCR in the presence of APAP (10 mM) for 48 h. (G) Western blot of autophagy-related proteins in L-02 cells supplemented with APAP (10 mM) for 48 h. (H) Quantification of Western blot. (I, J) Representative immunofluorescence images of mRFP-GFP-LC3 in L-02 cells supplemented with APAP (10 mM) for 48 h. Representative profiles of autophagosomes (RFP+GFP+dots) and autolysosomes (RFP+GFP-dots) per cell section, assessed by confocal microscopy are shown and were quantified. (K, L) Autophagic vacuoles (autophagosomes) were assessed by transmission electron microscopy (TEM) plus APAP (10 mM) for 48 h in L-02 cells. Representative TEM images are shown and typical autophagosomes are marked with black arrows. Autophagosome numbers per cell were calculated by counting the number of double-membrane organelles in 10 cells. Data are expressed as mean ± SEM (n = 3). **P < 0.01, ***P < 0.001 compared to control; # < 0.05, ### < 0.001 compared to APAP.
Pco Pixelfly, supplied by Excelitas corp, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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hep g2  (ATCC)
99
ATCC hep g2
Fisetin (FST) attenuates acetaminophen (APAP)-induced cytotoxicity by promoting autophagy and inhibiting inflammasome activation in L-02 cells. (A, B) Cell viability was assessed by MTT assay in response to APAP (10 mM) or N-acetyl-p-benzoquinoneimine (NAPQI) (200 μM) exposure for different periods in L-02 cells. (C, D) Cell viability was assessed by MTT assay in response to APAP (10 mM) or NAPQI (200 μM) exposure for 48 h in <t>HepaRG</t> cells. (E) Cell viability was assessed by MTT assay for different concentration of FST in L-02 cells. (F) Messenger RNA (mRNA) expression of ATG genes was determined by RT-PCR in the presence of APAP (10 mM) for 48 h. (G) Western blot of autophagy-related proteins in L-02 cells supplemented with APAP (10 mM) for 48 h. (H) Quantification of Western blot. (I, J) Representative immunofluorescence images of mRFP-GFP-LC3 in L-02 cells supplemented with APAP (10 mM) for 48 h. Representative profiles of autophagosomes (RFP+GFP+dots) and autolysosomes (RFP+GFP-dots) per cell section, assessed by confocal microscopy are shown and were quantified. (K, L) Autophagic vacuoles (autophagosomes) were assessed by transmission electron microscopy (TEM) plus APAP (10 mM) for 48 h in L-02 cells. Representative TEM images are shown and typical autophagosomes are marked with black arrows. Autophagosome numbers per cell were calculated by counting the number of double-membrane organelles in 10 cells. Data are expressed as mean ± SEM (n = 3). **P < 0.01, ***P < 0.001 compared to control; # < 0.05, ### < 0.001 compared to APAP.
Hep G2, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 99 stars, based on 1 article reviews
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99
ATCC human hepatocellular carcinoma cell lines heparg
Fisetin (FST) attenuates acetaminophen (APAP)-induced cytotoxicity by promoting autophagy and inhibiting inflammasome activation in L-02 cells. (A, B) Cell viability was assessed by MTT assay in response to APAP (10 mM) or N-acetyl-p-benzoquinoneimine (NAPQI) (200 μM) exposure for different periods in L-02 cells. (C, D) Cell viability was assessed by MTT assay in response to APAP (10 mM) or NAPQI (200 μM) exposure for 48 h in <t>HepaRG</t> cells. (E) Cell viability was assessed by MTT assay for different concentration of FST in L-02 cells. (F) Messenger RNA (mRNA) expression of ATG genes was determined by RT-PCR in the presence of APAP (10 mM) for 48 h. (G) Western blot of autophagy-related proteins in L-02 cells supplemented with APAP (10 mM) for 48 h. (H) Quantification of Western blot. (I, J) Representative immunofluorescence images of mRFP-GFP-LC3 in L-02 cells supplemented with APAP (10 mM) for 48 h. Representative profiles of autophagosomes (RFP+GFP+dots) and autolysosomes (RFP+GFP-dots) per cell section, assessed by confocal microscopy are shown and were quantified. (K, L) Autophagic vacuoles (autophagosomes) were assessed by transmission electron microscopy (TEM) plus APAP (10 mM) for 48 h in L-02 cells. Representative TEM images are shown and typical autophagosomes are marked with black arrows. Autophagosome numbers per cell were calculated by counting the number of double-membrane organelles in 10 cells. Data are expressed as mean ± SEM (n = 3). **P < 0.01, ***P < 0.001 compared to control; # < 0.05, ### < 0.001 compared to APAP.
Human Hepatocellular Carcinoma Cell Lines Heparg, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/heparg+human+hepatoma+cell+line/pm37121360-58-0-7?v=ATCC
Average 99 stars, based on 1 article reviews
human hepatocellular carcinoma cell lines heparg - by Bioz Stars, 2026-08
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Image Search Results


Fisetin (FST) attenuates acetaminophen (APAP)-induced cytotoxicity by promoting autophagy and inhibiting inflammasome activation in L-02 cells. (A, B) Cell viability was assessed by MTT assay in response to APAP (10 mM) or N-acetyl-p-benzoquinoneimine (NAPQI) (200 μM) exposure for different periods in L-02 cells. (C, D) Cell viability was assessed by MTT assay in response to APAP (10 mM) or NAPQI (200 μM) exposure for 48 h in HepaRG cells. (E) Cell viability was assessed by MTT assay for different concentration of FST in L-02 cells. (F) Messenger RNA (mRNA) expression of ATG genes was determined by RT-PCR in the presence of APAP (10 mM) for 48 h. (G) Western blot of autophagy-related proteins in L-02 cells supplemented with APAP (10 mM) for 48 h. (H) Quantification of Western blot. (I, J) Representative immunofluorescence images of mRFP-GFP-LC3 in L-02 cells supplemented with APAP (10 mM) for 48 h. Representative profiles of autophagosomes (RFP+GFP+dots) and autolysosomes (RFP+GFP-dots) per cell section, assessed by confocal microscopy are shown and were quantified. (K, L) Autophagic vacuoles (autophagosomes) were assessed by transmission electron microscopy (TEM) plus APAP (10 mM) for 48 h in L-02 cells. Representative TEM images are shown and typical autophagosomes are marked with black arrows. Autophagosome numbers per cell were calculated by counting the number of double-membrane organelles in 10 cells. Data are expressed as mean ± SEM (n = 3). **P < 0.01, ***P < 0.001 compared to control; # < 0.05, ### < 0.001 compared to APAP.

Journal: Frontiers in Pharmacology

Article Title: Fisetin Prevents Acetaminophen-Induced Liver Injury by Promoting Autophagy

doi: 10.3389/fphar.2020.00162

Figure Lengend Snippet: Fisetin (FST) attenuates acetaminophen (APAP)-induced cytotoxicity by promoting autophagy and inhibiting inflammasome activation in L-02 cells. (A, B) Cell viability was assessed by MTT assay in response to APAP (10 mM) or N-acetyl-p-benzoquinoneimine (NAPQI) (200 μM) exposure for different periods in L-02 cells. (C, D) Cell viability was assessed by MTT assay in response to APAP (10 mM) or NAPQI (200 μM) exposure for 48 h in HepaRG cells. (E) Cell viability was assessed by MTT assay for different concentration of FST in L-02 cells. (F) Messenger RNA (mRNA) expression of ATG genes was determined by RT-PCR in the presence of APAP (10 mM) for 48 h. (G) Western blot of autophagy-related proteins in L-02 cells supplemented with APAP (10 mM) for 48 h. (H) Quantification of Western blot. (I, J) Representative immunofluorescence images of mRFP-GFP-LC3 in L-02 cells supplemented with APAP (10 mM) for 48 h. Representative profiles of autophagosomes (RFP+GFP+dots) and autolysosomes (RFP+GFP-dots) per cell section, assessed by confocal microscopy are shown and were quantified. (K, L) Autophagic vacuoles (autophagosomes) were assessed by transmission electron microscopy (TEM) plus APAP (10 mM) for 48 h in L-02 cells. Representative TEM images are shown and typical autophagosomes are marked with black arrows. Autophagosome numbers per cell were calculated by counting the number of double-membrane organelles in 10 cells. Data are expressed as mean ± SEM (n = 3). **P < 0.01, ***P < 0.001 compared to control; # < 0.05, ### < 0.001 compared to APAP.

Article Snippet: The human hepatocellular carcinoma cell line HepaRG ( ) was purchased from Merck Millipore (Burlington, MA, USA).

Techniques: Activation Assay, MTT Assay, Concentration Assay, Expressing, Reverse Transcription Polymerase Chain Reaction, Western Blot, Immunofluorescence, Confocal Microscopy, Transmission Assay, Electron Microscopy, Membrane, Control