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Image Search Results
Journal: Frontiers in Pharmacology
Article Title: Fisetin Prevents Acetaminophen-Induced Liver Injury by Promoting Autophagy
doi: 10.3389/fphar.2020.00162
Figure Lengend Snippet: Fisetin (FST) attenuates acetaminophen (APAP)-induced cytotoxicity by promoting autophagy and inhibiting inflammasome activation in L-02 cells. (A, B) Cell viability was assessed by MTT assay in response to APAP (10 mM) or N-acetyl-p-benzoquinoneimine (NAPQI) (200 μM) exposure for different periods in L-02 cells. (C, D) Cell viability was assessed by MTT assay in response to APAP (10 mM) or NAPQI (200 μM) exposure for 48 h in HepaRG cells. (E) Cell viability was assessed by MTT assay for different concentration of FST in L-02 cells. (F) Messenger RNA (mRNA) expression of ATG genes was determined by RT-PCR in the presence of APAP (10 mM) for 48 h. (G) Western blot of autophagy-related proteins in L-02 cells supplemented with APAP (10 mM) for 48 h. (H) Quantification of Western blot. (I, J) Representative immunofluorescence images of mRFP-GFP-LC3 in L-02 cells supplemented with APAP (10 mM) for 48 h. Representative profiles of autophagosomes (RFP+GFP+dots) and autolysosomes (RFP+GFP-dots) per cell section, assessed by confocal microscopy are shown and were quantified. (K, L) Autophagic vacuoles (autophagosomes) were assessed by transmission electron microscopy (TEM) plus APAP (10 mM) for 48 h in L-02 cells. Representative TEM images are shown and typical autophagosomes are marked with black arrows. Autophagosome numbers per cell were calculated by counting the number of double-membrane organelles in 10 cells. Data are expressed as mean ± SEM (n = 3). **P < 0.01, ***P < 0.001 compared to control; # < 0.05, ### < 0.001 compared to APAP.
Article Snippet: The human
Techniques: Activation Assay, MTT Assay, Concentration Assay, Expressing, Reverse Transcription Polymerase Chain Reaction, Western Blot, Immunofluorescence, Confocal Microscopy, Transmission Assay, Electron Microscopy, Membrane, Control